![]() ![]() Currently, no integrated, optimized, and high-throughput methylation-specific primer design software methods are available for both BS- and MSRE-based methods. Designing specific and optimized primers for target regions is the most critical and challenging step in obtaining the adequate DNA methylation results using PCR-based methods. ![]() Thus, an integrated approach would be extremely useful to quantify the DNA methylation status successfully with great sensitivity and specificity. However, both methods have advantages and limitations. Bisulfite (BS) conversion-based and methylation-sensitive restriction enzyme (MSRE)-based PCR methods have been the most commonly used techniques for locus-specific DNA methylation analysis.
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